Insulin-like Growth Factor(IGF-I) has the potent chemotactiv and proliferative effect on preosteoblasts and hematocytes. There were many researches on bone cells, but now there are insufficient data of the effect of IGF-I on the periodontal
ligament(PDL) cells.
The purpose of this study is to evaluate the effects of IGF-I on the PDL cells, Human PDL cells were prepared from the first premolar tooth extracted for the orthodontic treatment and were cultured in DMEM/10% FBS at a CO2 incubator(37℃).
The concentrations of IGF-I were as follows: 3ng/ml, 6ng/ml, 25ng/ml, 50ng/ml, 100ng/ml and 150ng/ml. In the IGF-1 group. cell numbers were estimated by counting cells under the microscope at 2 days after. Protein determination was made by the
method of
Lowery et al.
@ES The observed results were as follows:
@EN 1 The IGF-I group showed the concentration-dependent increase of cell numbers at 2 days after. IGF-I. stimulated significantly PDL mitosis at all concentrations.
2. The maximum effect of PDL proliferation was observed at the concentration of 25ng/ml IGF-I. Its effect was about twofold tha that of the control group. At that concentration, cell numbers were(12.28±1.10)×10E4 cell/ml
3. Protein contents of PDL cells was increased in all experimental groups and the degree of increase in protein contents was highest at concentration of 25ng/ml IGF-I
|